1. Naradun N., Chueakwon P., Kamkaew A., Hsu K.Y., Lee M.N., Chansaenpak K., Wang Y.S.* & Lai R.I.*, “Ferritin Fused with miniSOG for In Vitro Photodynamic Therapy”, 2025, ACS Appl. Bio Mater. 8, 3909–3919
  2. Tsou J.C.†, Tsou C.J.†, Wang C.H.†, Ko A.L.A.†, Wang Y.H., Liang H.H., Sun J.C., Huang K.F., Ko T.P., Lin S.Y. & Wang Y.S.*, “Site-specific histidine aza-Michael addition in proteins enabled by a ferritin-based metalloenzyme”, 2024. J. Am. Chem. Soc. 146, 33309–33315. (Featured as the Supplementary Cover of the issue, News reported by Natural Science Library Service Project: Chemistry Division, January, 2025, and Research Highlights at National Synchrotron Radiation Research Center website, January, 2025.)
  3. Jiang H.K.†, Weng J.H.†, Wang Y.H.†, Tsou J.C., Chen P.J., Ko A.L.A., Söll D., Tsai M.D. & Wang Y.S.*, “Rational design of the genetic code expansion toolkit for in vivo encoding of D-amino acids”, 2023 Front. Genet. 14:1277489.
  4. Jiang H.K., Ambrose N., Chung C., Wang Y.S., Söll D.* & Tharp J.M.*, “Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression”, 2023 Proc. Natl. Acad. Sci. U.S.A. 120, e2219758120.
  5. Guo L.T.†, Amikura K.†, Jiang H.K.†, Mukai, T., Fu X., Wang Y.S., O’Donoghue P., Söll D.* & Tharp J.M.*, “Ancestral archaea expanded the genetic code with pyrrolysine”, 2022 J. Biol. Chem. 298:102521.
  6. Wang D., Kao M.R., Li J., Sun P., Meng Q., Vyas A., Liang P. H., Wang Y.S. & Hsieh Y.S.Y.*, “Novel two-step process in cellulose depolymerization: hematite-mediated photocatalysis by lytic polysaccharide monooxygenase and fenton reaction”, 2022 J. Agric. Food Chem. 70, 9941–9947.
  7. Wang Y.H.†, Jian M.L.†, Chen P.J., Tsou J.C., Truong, L.P. & Wang Y.S.*, “Ferritin conjugates with multiple clickable amino acids encoded by C-terminal engineered pyrrolysyl-tRNA synthetase”, 2021 Front. Chem. 9:779976.
  8. Jiang H.K.†, Wang Y.H.†, Weng J.H.†, Lee M.N., Li C.L., Kurkute P., Chen P.J., Tseng H.W., Tsai M.D. & Wang Y.S.*, “Probing the active site of deubiquitinase USP30 with non-canonical tryptophan analogues”, 2020 Biochemistry 59, 2205–2209. (Selected as the Supplementary Cover of the issue, and mentioned in ACS Axial as the most read article in June 2020 in Biochemistry.)
  9. Jiang H.K., Lee M.N., Tsou C.J., Chang K.W. Tseng H.W., Chen K.P., Li Y.K. & Wang Y.S.*, “Linker and N-terminal domain engineering of pyrrolysyl-tRNA synthetase for substrate range shifting and activity enhancement”, 2020 Front. Bioeng. Biotechnol. 8:235.
  10. Tseng H.W., Baumann T., Sun H., Wang Y.S., Ignatova Z. & Budisa N.*, “Expanding the scope of orthogonal translation with pyrrolysyl-tRNA synthetase dedicated to aromatic amino acids”, 2020 Molecules 25, 4418.
  11. Jiang H.K.†, Kurkute P.†, Li C.L., Wang Y.H., Chen P.J., Lin S.Y. & Wang Y.S.*, “Revealing USP7 deubiquitinase substrate specificity by unbiased synthesis of ubiquitin tagged SUMO2”, 2020 Biochemistry 59, 3796–3801. (Selected as the front cover of the issue.)
  12. Wang Y.S.*, Wu K.P.*, Kurkute P., Jiang H.K. & Chen R.H.* “Branched ubiquitination: detection methods, biological functions and chemical synthesis”, 2020 Molecules 25, 5200.
  13. Englert M., Vargas-Rodriguez, O., Reynolds N.M., Wang Y.S., Söll D.* & Umehara T.*, “A genomically modified Escherichia coli strain carrying an orthogonal E. coli histidyl-tRNA synthetase-tRNAHis pair”, 2017 BBA-Gen. Subjects 1861, 3009–3015.
  14. Suzuki T., Miller C., Guo L.T., Ho J.M.L., Bryson D.I., Wang Y.S., Liu D.R. & Söll D.*, “Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase”, 2017 Nat. Chem. Biol. 13, 1261–1266.
  15. harma V., Wang Y.S. & Liu W.R.*, “Probing the catalytic charge-relay system in alanine racemase with genetically encoded histidine mimetics”, 2016 ACS Chem. Biol. 11, 3305–3309.
  16. Amiram M., Haimovich A.D., Fan C.Q., Wang Y.S., Aerni H.R., Ntai I., Moonan D.W., Ma N.J., Rovner A.J., Hong S.H., Kelleher N.L., Goodman A.L., Jewett M.C., Söl, D.*, Rinehart J.* & Isaacs F.J.*, “Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids”, 2015 Nat. Biotechnol. 33, 1272–1279.
  17. Englert M.†, Nakamura A.†, Wang Y.S.†, Söll D.* & Guo L.T.*, “Probing the active site tryptophan of Staphylococcus aureus thioredoxin with an analog”, 2015 Nucleic Acids Res. 43, 11061–11067.
  18. Guo L.T.†, Wang Y.S.†, Nakamura A.†, Eiler D.†, Kavran J.M., Wong M., Kiessling L.L., Steitz T.A., O’Donoghue P.* & Söll, D.*, “Polyspecific pyrrolysyl-tRNA synthetases from directed evolution”, 2014 Proc. Nalt. Acad. Sci. USA 111, 16724–16729.
  19. Tharp J.M., Wang Y.S., Lee Y.J., Yang Y.Y. & Liu W.R.*, “The genetic incorporation of seven ortho-substituted phenylalanine derivatives”, 2014 ACS Chem. Biol. 9, 884–890.
  20. Tuley A., Wang Y.S., Fang X. Q., Kurra Y., Reznom Y.H. & Liu W.R.*, “The genetic incorporation of thirteen novel non-canonical amino acids”, 2014 Chem. Commun. 50, 2673–2675.
  21. Fan C.Q., Ho J.M.H., Chirathivat N., Söll D.* & Wang Y.S.* “Exploring the substrate range of wild-type aminoacyl-tRNA synthetases”, 2014 ChemBioChem 15, 1805–1809.
  22. Wang Y.S., Fang X., Chen H.Y., Wang Z., Wu B., Hilty C.B. & Liu, R.W.*, “Genetic incorporation of twelve meta-substituted phenylalanine derivatives using a single pyrrolysyl-tRNA synthetase mutant”, 2013 ACS Chem. Biol. 8, 405–415.
  23. Ko J.H.†, Wang Y.S.†, Nakamura A.†, Guo L.T., Söll D.* & Umehara T.*, “Pyrrolysyl-tRNA synthetase variants reveal ancestral aminoacylation function”, 2013 FEBS Lett. 578, 3243–3248. (Featured on the cover of the issue.)
  24. O’Donoghue P.†, Ling J.†, Wang Y.S.† and Söll D.*, “Upgrading protein synthesis for synthetic biology”, 2013 Nat. Chem. Biol. 9, 594–598.
  25. Wang Y.S., Fang X., Wallace A.L., Wu B. & Liu R.W.*, “A rationally designed pyrrolysyl-tRNA synthetase mutant has a broad substrate specificity”, 2012 J. Am. Chem. Soc. 134, 2950–2953.
  26. Wang Z.†, Wang Y.S.†, Pai P.J., Russell W.K., Russell D.H. & Liu R.W.*, “A facile method to synthesize histones with posttranslational modification mimics”, 2012 Biochemistry 51, 5232–5234.
  27. Liu R.W.*, Wang Y.S. & Wan W., “Synthesis of proteins with defined posttranslational modifications using the genetic noncanonical amino acid incorporation approach”, 2011 Mol. Biosyst. 7, 38–47. (An invited review article for the themed collection of “Post-translational Modifications”, and a featured article in the “2011 Review Articles in Chemical Biology” in Mol. Biosys.)
  28. Wang Y.S., Russell W.K., Wang Z.Y., Wan W., Dodd L.E., Pai P.J., Russell D.H. & Liu, R.W.*, “The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of L-phenylalanine and its derivatives”, 2011 Mol. Biosyst. 7, 714–716. (Mentioned as top 10% of the most cited articles in Mol. Biosys., 2011–2012.)
  29. Wang Y.S., Wu B., Wang Z., Huang Y., Wan W., Russell W.K., Pai P.J., Moe Y.N., Russell D.H. & Liu R.W.*, “A genetically encoded photocaged N-methyl-L-lysine”, 2010 Mol. Biosyst. 6, 1557–1560.
  30. Ng K.Y., Tu L.C., Wang Y.S., Chan S.I. & Yu S.S.F.*, “Probing the hydrophobic pocket of the active site in the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) by variable stereoselective alkane hydroxylation and olefin epoxidation”, 2008 ChemBioChem 9, 1116–1123.
  31. Huang D.S.* Whang T.J., Cheng F.C., Wu, Y.P., Wang Y.T., Luo W.I. & Wang Y.S., “Toxicity assessment of mono‐substituted benzenes and phenols using a Pseudomonas initial oxygen uptake assay”, 2005 Environ. Toxicol. Chem. 24, 253–260.
  32. Whang T.J., Wang Y.T., Wu Y.P., Wang Y.S., Tsai M.C. & Huang D.S.*, “Assessment of baseline toxicity of mono-cyclic aromatic compounds by Pseudomonas initial oxygen uptake assay”, 2005 SAR and QSAR in Environ. Res. 16, 247–262.
  33. Yu S.S.F., Chen K.H.C., Tseng M.Y.H., Wang Y.S., Tseng C.F., Chen Y.J., Huang D.S.* & Chan, S. I.*, “Production of high-quality particulate methane monooxygenase in high yields from Methylococcus capsulatus (Bath) with a hollowfiber membrane bioreactor”, 2003 J. Bact. 185, 5915–5924.
  34. Huang D.S.*, Wu S.H., Wang Y.S., Yu S.S.F. & Chan S.I.*, “Determination of the carbon kinetic isotope effects on propane hydroxylation mediated by the methane monooxygenases from Methylococcus capsulatus (Bath) by using stable carbon isotopic analysis”, 2002 ChemBioChem 3, 760–765
  35. Wan W., Wang Y.S. & Liu R.W.*, “Genetically encoding bioorthogonal functional groups for site-selective protein labeling”, 2012 Organic Chem. Curr. Res. 1(3):1:e111.

名稱 開始日期 結束日期 委託單位 編號 參與身份別
新穎蛋白麥克連接酶設計優化與抗登革熱病毒抗體定點修飾研究(1/3) (The Novel Michael Ligase Design and Its Application for Site-specific Chemical Modifications of Dengue Virus Neutralizing Antibody 1/3) 2025/08/01 2026/07/31 國科會 NSTC 114-2113-M-003-019 主持人
含非典型胺基酸蛋白做為探測蛋白交互作用及聚泛素拓樸學(2/2) (Genetically encoded non-canonical amino acid for probing protein-protein interactions and polyubiquitin topology 2/2) 2024/08/01 2025/07/31 國科會 NSTC 113-2113-M-001-006 主持人
含非典型胺基酸蛋白做為探測蛋白交互作用及聚泛素拓樸學(1/2) (Genetically encoded non-canonical amino acid for probing protein-protein interactions and polyubiquitin topology 1/2) 2023/08/01 2024/07/31 國科會 NSTC 112-2113-M-001-015 主持人
含非典型胺基酸嵌入蛋白設計及藥物鍵結複合蛋白應用 (Protein design using genetic code expansion and protein-drug conjugates applications) 2022/08/01 2023/07/31 國科會 MOST 111-2113-M-001-039 主持人
體內D型非典型胺基酸嵌入平台建立及篩選混合鏡像胜肽及蛋白藥物應用 (Genetically encoded D-noncanonical amino acids development and their design in heterochiral peptide and protein therapeutics) 2021/08/01 2922/07/31 國科會 MOST 110-2113-M-001-044 主持人
體內非典型胺基酸嵌入酵素功能性探究及新穎功能蛋白設計應用 (Enzyme Functional Manipulation with Genetically Encoded Noncanonical Amino Acids and Novel Functional Protein Design) 2018/08/01 2021/07/31 國科會 MOST 107-2113-M-001-025-MY3 主持人

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